Real Time PCR (QPCR)

Real-time PCR is a highly sensitive and highly accurate means of quantifying particular RNAs, and is especially well suited to validating microarray (e.g. Affymetrix) results.

Real-time PCR requires specially designed PCR primers that can be used with a fluorescent dye (SYBR Green) or a combination of two flanking primers and an interior primer linked to fluorescent tag and quencher molecules (Taqman probe). These probes are available pre-made and pre-tested for most mouse and human genes.

Real-time PCR can also be used for measuring Single Nucleotide Polymorphisms, or SNPs, or for specialized assays such as alternative RNA splicing assays.

The ATG Facility performs quantitative real-time PCR assays in 384 well plates, which saves reagents and sample.

Please contact the ATG Facility staff for more information about assays and pricing.

Technical Director:
Gavin Pickett, Ph.D.
CRF G-21
Tel: (505) 272-0680

Jason Byars
CRF G-21
Tel: (505) 272-0675

Senior Technical Staff:
Jamie Padilla, M.S.
CRF 118
Tel: (505) 272-5564

Jennifer Woods
CRF 121
Tel: (505) 272-3464

Faculty Director:
Scott A. Ness, Ph.D.
Professor, Internal Medicine
Office: CRF 121; Tel: (505) 272-9883